L-alanine transport in isolated cells of interscapular brown adipose tissue in rat.
نویسندگان
چکیده
The pattern of L-alanine uptake in isolated cells of interscapular brown adipose tissue has been determined. The uptake can be divided into the diffusion component (Kd = 0.55 min-1) and a saturable Na(+)-dependent transport (KM = 0.87 mM and Vmax = 155 nmol/min/10(6) cells). The saturable component can be subdivided into MeAIB-sensitive (KM = 1.63 mM and Vmax = 162 nmol/min/10(6) cells) and MeAIB-insensitive (KM = 3.2 mM and Vmax = 39.5 nmol/min/10(6) cells). This kinetic pattern could indicate the presence of transport system (s) that resemble the commonly described transport systems for alanine uptake in several tissues.
منابع مشابه
Growth Kinetics and in Vitro Aging of Mesenchymal Stem Cells Isolated From Rat Adipose Versus Bone Marrow Tissues
Objective- To investigate and compare growth potential as well as aging of mesenchymal stem cells (MSCs) derived from rat bone marrow tissue and adipose tissue (AT) occurred at epicardial and epididymal regions. Design- Experimental study. Animals- 10 Wistar Rats. Procedures- Rat MSCs occurred at bone marrow and epicardial and epididymal AT were isolated and culture expanded through sev...
متن کاملMesenchymal Stem Cells Derived from Rat Epicardial Versus Epididymal Adipose Tissue
Objective(s) Some investigation has indicated that adipose-derived stem cells possess different surface epitopes and differentiation potential according to the localization of fat pad from which the cells were derived. In the present study proliferation capacity and aging of such cells were explored. Materials and Methods Adherent cells were isolated from the collagenase digests of adipose tiss...
متن کاملSkin wound healing following the spray of human abdominal adipose tissue-derived mesenchymal stem cells in diabetic male rat
Introduction: Diabetes is rising worldwide and impaired wound healing is one of its major complications. This study aimed to determine the effects of adipose-derived mesenchymal stem cells (MSCs) on wound healing in diabetic rats. Materials and Methods: In this experimental study, abdominal adipose tissue was obtained from 10 patients who underwent an abdominoplasty. MSCs were isolated from adi...
متن کاملEvidence that noradrenaline increases pyruvate dehydrogenase activity and decreases acetyl-CoA carboxylase activity in rat interscapular brown adipose tissue in vivo.
The rate of fatty acid synthesis in interscapular brown adipose tissue of female cold-adapted rats, as measured by the incorporation of 3H from 3H2O into tissue lipid, was decreased by about 70% after injection of noradrenaline. There was a similar decrease in the activity of acetyl-CoA carboxylase. In contrast, the proportion of pyruvate dehydrogenase in its active non-phosphorylated form was ...
متن کاملLeptin immunoexpression and innervation in rat interscapular brown adipose tissue of cold-acclimated rats: the effects of L-arginine and L-NAME.
The aim of the present study was to explore the effect of nitric oxide on leptin immunoexpression and innervation in interscapular brown adipose tissue (IBAT) of room- and cold- acclimated rats. Animals acclimated both to room-temperature (22 +/- 1 degrees C) and cold (4 +/- 1 degrees C) were treated with L-arginine, a substrate for nitric oxide synthases (NOSs), or N?-nitro-L-arginine methyl e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Bioscience reports
دوره 11 2 شماره
صفحات -
تاریخ انتشار 1991